Short Communication
Spanish population data and forensic usefulness of a novel Y-STR set (DYS437, DYS438, DYS439, DYS460, DYS461, GATA A10, GATA C4, GATA H4)
María T. Zarrabeitia1 , José A. Riancho2, Leonor Gusmão4, María V. Lareu3, Carolina Sañudo1, António Amorim4 and Angel Carracedo3

(1) Unidad de Medicina Legal, Facultad de Medicina, Universidad de Cantabria C/Herrera Oria, s/n, 39011 Santander, Spain
(2) Universidad de Cantabria, Departamento de Medicina Interna, Santander, Spain
(3) Universidad de Santiago, Instituto de Medicina Legal, Santiago de Compostela, Spain
(4) Universidad do Porto, Instituto de Patologia e Imunologia Molecular, Porto, Portugal

María T. Zarrabeitia
Phone: +34-942-201984
Fax: +34-942-201903

Received: 13 September 2002 Accepted: 24 June 2003 Published online: 2 August 2003

Abstract DNA typing of 8 recently described STRs on the Y chromosome was carried out by means of 2 multiplex amplification reactions for 134 unrelated males from Cantabria, a region in northern Spain. Multiplex 1 included loci DYS460 (GATA A7.1), GATA A10, GATA H4 and DYS439; multiplex 2 included DYS461 (GATA A7.2), GATA C4, DYS437 and DYS438. Haplotype diversity was found to be 99.36%, similar to that obtained with the standard 9-STR set ("minimal haplotype") of the European Y-user group (99.35%). The 13-locus haplotype resulting from the combination of the standard minimal haplotype and the 4-locus multiplex 1 showed a 99.89% diversity. Further inclusion of the 4 loci in multiplex 2 resulted in a haplotype diversity of 99.93%. The combination of the "minimal haplotype" and the multiplex 1 in the present study may be an efficient way of increasing the power of discrimination in forensic cases.