Int J Legal Med. 1999;112(6):403-5.

Haplotype frequencies of eight Y-chromosome STR loci in Barcelona (North-East Spain).

Gene M, Borrego N, Xifro A, Pique E, Moreno P, Huguet E.

Forensic Genetics Laboratory, Department of Legal Medicine, Faculty of Medicine, University of Barcelona, C. Casanova 143, E-08036 Barcelona, Spain.

Haplotype frequencies for eight Y-chromosomal short tandem repeat (STR) loci were determined in paragraph signa population sample from Barcelona (NE Spain). After PCR amplification and denaturing PAGE electrophoresis, DYS19, DYS388, DYS389 I/II, DYS390, DYS391, DYS392 and DYS393 loci were typed. Complete eight paragraph signY-chromosomal STRs haplotypes could be formed for 223 subjects, among which 137 different haplotypes were observed. The most common haplotype was shared by 13% of the sample, while 108 haplotypes were unique. The discrimination capacity was 61.5% and the gene diversity was 0.978. From the forensic point of view the combined polymorphisms provide a high diagnostic efficiency.