Med Pregl. 2001 May-Jun;54(5-6):224-8.

[Distribution of HLA class II antigens and allele linkage disequilibrium in the population of Vojvodina]

Vojvodic S, Belic B.


INTRODUCTION: Polymorphism of human leucocyte antigens (HLA) is important in transplantation medicine, anthropological studies and paternity testing. MATERIAL AND METHODS: We investigated polymorphisms of HLA class II in population of Vojvodina by means of serologic typing using microlymphocitotoxicity test. We calculated the HLA-DR and HLA-DQ gene frequencies in 174 subjects. Haplotype frequencies, coefficient of linkage disequilibrium (delta values) and their statistical significant levels, were analyzed on the basis of these data. RESULTS: The most frequent HLA-DR and HLA-DQ alleles were: DR5(11) (gene frequency 0.138), DR4 (gene frequency 0.083), DR1 (gene frequency 0.077), DQ1 (gene frequency 0.388), DQ3 (gene frequency 0.197), and DQ2 (gene frequency 0.09). The highest positive values of coefficient of linkage disequilibrium (delta) were calculated for the following haplotypes: DR1DQ1 (delta value 0.07308708), DR2DQ1 (delta value 0.059846528), DR3DQ2 (delta value 0.06193263), DR2(15)DQ1 (delta value 0.039186022), DR5(11)DQ3(7) (delta value 0.04969439), DR7DQ2 (delta value 0.057985517). Significant differences between observed vs. expected haplotype frequencies were also considered for these haplotypes. CONCLUSION: This study indicates distinctiveness and specificity of the population of Vojvodina and highlights the importance of determining HLA frequencies in endogamic groups of Serbia.